Biological Safety Manual
The University of North Carolina at Chapel Hill
Environment, Health and Safety Office
August 2000
Environment, Health and Safety Office
August 2000
To request a paper copy of the complete manual, please call EHS at 962-5507.
Table of Contents
- Chapter 1 Introduction
- Chapter 2 Occupational Hazards in Microbiological Laboratories
- Chapter 3 Principles of Biosafety
- Chapter 4 Description of Laboratory Biosafety Levels
- Chapter 5 Description of Vertebrate Animal Biosafety Levels
- Chapter 6 Risk Assessment
- Chapter 7 Recommended Biosafety Levels for Infectious Agents and Infected Animals
- Chapter 8 Agent Summary Statements
- Bacterial Agents
- Fungal Agents
- Parasitic Agents
- Prions
- Rickettsial Agents
- Viral Agents (other than arboviruses)
- Arboviruses and Related Zoonotic Viruses
- Chapter 9 Biosafety in Laboratories Handling Human Blood
- Chapter 10 Research Involving Recombinant DNA Molecules
- Chapter 11 Hazards of Cell and Tissue Culture Systems
- Chapter 12 Safe Handling of Laboratory Animals
- Chapter 13 Laboratory Techniques for Biohazard Control
- Chapter 14 Biological Safety Cabinets
- Chapter 15 Decontamination and Disposal
- Chapter 16 Clean Up of Biohazardous Spills
- Chapter 17 Transportation and Transfer of Biological Agents
- Chapter 18 Guidelines for Work With Toxins of Biological Origin
- Chapter 19 Laboratory Security and Emergency Response Planning
- App 15A Decontamination and Disposal of Infectious Waste
- Table 1 Comparison of Biological Safety Cabinet Characteristics and Applications

